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Atención integrada madre-niño. efectos sobre adherencia y conocimientos de métodos anticonceptivos
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Fotia Perniciaro, Lucrecia

; Sapienza, Cinthia

; Carrera Ramos, Patricia

; Fasano, María Victoria

; Méndez, Ignacio

; Malpeli, Agustina

Medicina (Buenos Aires)

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Ago 2023, Volume 83 Nº 4 Páginas 533 - 542

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen Introducción : La evidencia científica sugiere que es trategias de atención conjunta madre-hijo facilitarían el conocimiento de métodos anticonceptivos (MAC) y su acceso. El objetivo fue evaluar el efecto del Modelo de atención integrada de la madre y el niño sobre la adherencia y conocimientos de MAC en mujeres durante el primer semestre postparto. Métodos : Se realizó un estudio de intervención, se conformaron 2 grupos, GI: grupo de intervención (3 con troles hasta los 6 meses postparto) y GC: grupo control (un control a los 6 meses postparto). La intervención consistió en asesoramiento presencial acerca de MAC combinada con folletería informativa y mensajes de WhatsApp®. Se recabaron datos sociodemográficos, an tecedentes gineco-obstétricos, uso y conocimientos de MAC. Se comparó la adherencia al uso y el conocimiento de MAC en ambos grupos a los 6 meses post parto. El análisis se realizó mediante el software R versión 4.0.3. Resultados : Se incorporaron 39 mujeres en cada grupo. Se halló una diferencia en el uso de MAC entre grupos a los 6 meses (92.3% vs. 64.1%), siendo más eleva do su uso en el GI. Se hallaron diferencias significativas en el conocimiento de algunos MAC a los 6 meses. Un mayor porcentaje de mujeres del GI conocía las pastillas anticonceptivas (p = 0.009), ligadura de trompas (p = 0.04) y la vasectomía (p = 0.010), en comparación con el GC. Discusión : La intervención en el postparto temprano con diversas estrategias de comunicación e información pueden ser útiles para elegir el MAC que se considere más adecuado para cada mujer y su utilización correcta.

Abstract Introduction : Scientific evidence suggests that moth er-child joint care strategies would facilitate knowledge of contraceptive methods (MAC) and their access. The objective was to evaluate the effect of the Integrated Mother and Child Care Model on adherence and knowl edge of CAM in women during the first postpartum semester. Methods : An intervention study was carried out, 2 groups were formed, GI: intervention group (3 controls up to 6 months postpartum) and GC: control group (one control at 6 months postpartum). The intervention con sisted of face-to-face counseling about MAC combined with informative brochures and WhatsApp® messages. Sociodemographic data, gynecological and obstetric history, use and knowledge of contraceptive methods were collected. Adherence to the use and knowledge of MAC were compared in both groups at 6 months post partum. The analysis was performed using R software version 4.0.3. Results : Thirty-nine women were incorporated into each group. A difference was found in the use of MAC between groups at 6 months (92.3% vs. 64.1%), its use being higher in GI. Significant differences were found in the knowledge of some MAC at 6 months. A higher percentage of women in GI knew about birth control pills (p = 0.009), tubal ligation (p = 0.04) and vasectomy (p = 0.010), compared to GC. Discussion : Early postpartum intervention with vari ous communication and information strategies can be useful to choose the MAC that is considered most ap propriate for each woman and its correct use.

Análisis de tesis médicas de pregrado en una universidad de Perú, 2011-2020: publicación en revistas indizadas y factores asociados
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Aragón-Ayala, Carlos Jesús

; Merma-Valero, Judith Marie

; Olivera-Zapana, Bruno

; Urday-Ramos, Patricia

; Turpo-Jove, Karlibeth

; Morron-Vizarreta, Adriana

; Quispe-Juli, Cender Udai

Revista Cubana de Información en Ciencias de la Salud

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2023, Volume 34 elocation e2172

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

ABSTRACT Quantitative factors associated with the publication of undergraduate medical theses have not been sufficiently studied. Therefore, the research described publication rates, as well as the association with thesis, advisor, and thesis writer characteristics. Undergraduate medical theses from the Universidad Nacional de San Agustín de Arequipa, Peru, during the period 2011-2020 were accessed virtually. Multiple logistic regression analysis was used to evaluate the association between thesis publication in indexed journals (Scopus) and the previous publication experience in indexed journals in Scopus of the advisor and the thesis author, the unit of analysis, sample size, study design, number of pages, approval by the research ethics committee and the gender of the thesis author. Out of 884 theses only 12 (1.5%) were published; this constitutes 1.23% of the theses published annually, with a constant trend. The previous publication experience of the advisor was associated with the placement of theses in indexed journals (OR = 8.97 [1.70-42.98]; p = 0.005) and in journals indexed to Scopus (OR = 14.64 [1.24-336.11]; p = 0.037). Presenting the approval of the institution's ethics committee was associated with publishing the thesis in journals indexed to Scopus (OR = 12.45 [1.06-285.94]; p = 0.050). Publication of undergraduate medical theses at this university is low and consistent. It was associated with having a thesis advisor with previous experience in publishing in Scopus-indexed journals and having approval by an ethics committee. There is an urgent need to implement strategies to increase thesis publication.

RESUMEN Los factores cuantitativos asociados a la publicación de tesis médicas en el pregrado no han sido lo suficientemente estudiados. Por ello, la investigación describió las tasas de publicación, así como la asociación con las características de las tesis, del asesor y del tesista. Se accedió, de manera virtual, a las tesis médicas de pregrado de la Universidad Nacional de San Agustín de Arequipa, Perú, durante el período 2011-2020. Mediante el análisis de regresión logística múltiple, se evaluó la asociación entre la publicación de la tesis en revistas indizadas (Scopus) y la experiencia previa en publicación en revistas indizadas en Scopus del asesor y del tesista, la unidad de análisis, el tamaño de muestra, diseño de estudio, número de páginas, aprobación por el comité de ética de investigación y el sexo del tesista. De 884 tesis solo 12 (1,5 %) se publicaron; lo que constituye el 1,23 % de las tesis publicadas anualmente, con tendencia constante. La experiencia previa en publicación del asesor se asoció con la colocación de las tesis en revistas indizadas (OR = 8,97 [1,70-42,98]; p = 0,005) y en revistas indizadas a Scopus (OR = 14,64 [1,24-336,11]; p = 0,037). Presentar la aprobación del comité de ética de la institución se asoció con publicar la tesis en revistas indizadas a Scopus (OR = 12,45 [1,06-285,94]; p = 0,050). La publicación de tesis médicas de pregrado en esta universidad es baja y constante. Se asoció con tener un asesor de tesis con experiencia previa en publicaciones en revistas indizadas a Scopus y a tener aprobación por comité de ética. Urge implementar estrategias para aumentar la publicación de tesis.

Comportamiento imagenológico de las deformidades faciales
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Blanco Moredo, Elizabeth

; Gutiérrez Hernández, María Elena

; Valdés Collazo, Carlos

; Pérez Ramos, Patricia

; Dunán Mesa, Lázaro Yunier

MediSur

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Jun 2022, Volume 20 Nº 3 Páginas 432 - 440

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

ABSTRACT Background For the surgical management of the patient with facial deformity, the use of the clinical and radiographic examination of each case is vital, since depending on the findings, a decision is made between the various therapeutic alternatives available in this field. Objective to describe the imaging behavior of facial deformities in adult patients. Methods a descriptive cross-sectional study was carried out in 46 patients older than 18 years old, with a diagnosis of facial deformity, who attended the Maxillofacial Surgery Service, Calixto García Hospital, from November 2017 to November 2019. The analyzed variables were: type of tissue, magnitude, and plane in which the alterations were evidenced. Fisher's exact test was used, with a confidence level of 95 %, to determine the analytical relationships between the variables; as well as Kendall's tau b and Spearman's Rho rates. Results The 45.7 % of the deformities presented in both planes; 50 % of them with magnitudes between 11-30 mm; and in 41.3 % of the patients they affected the soft tissue. The correlations between the distribution of patients and the sets of variables plane/tissue type and plane/magnitude were weakly negative; while tissue type/magnitude presented a very significant moderate positive correlation, with significance values between 0.001 and 0.003. Conclusion soft tissue deformities predominated, in both planes and with magnitudes between 11-30 mm. The imaging variables studied showed an interdependent behavior.

RESUMEN Fundamento Para el manejo quirúrgico del paciente con deformidad facial resulta vital el uso del examen clínico y radiográfico de cada caso, pues en dependencia de los hallazgos se decide entre las diversas alternativas terapéuticas disponibles en este campo. Objetivo describir el comportamiento imagenológico de las deformidades faciales en pacientes adultos. Métodos se realizó un estudio descriptivo de corte transversal, en 46 pacientes mayores de 18 años, con diagnóstico de deformidad facial, los cuales acudieron al Servicio de Cirugía Maxilofacial, del Hospital Calixto García, en el período comprendido desde noviembre de 2017 a noviembre de 2019. Se analizaron las variables: tipo de tejido, magnitud, y plano en el que se evidenciaron las alteraciones. Se utilizó la prueba exacta de Fisher, con un nivel de confianza al 95 %, para determinar las relaciones analíticas entre las variables; así como los índices tau b de Kendall y el Rho de Spearman. Resultados el 45,7 % de las deformidades se presentó en ambos planos; el 50 % de ellas con magnitudes entre 11-30 mm; y en el 41,3 % de los pacientes afectaron al tejido blando. Las correlaciones entre la distribución de pacientes y los conjuntos de variables plano/tipo de tejido y plano/magnitud resultaron negativas débiles; mientras que tipo de tejido/magnitud presentó una correlación positiva moderada muy significativa, con valores de significación entre 0,001 a 0,003. Conclusión predominaron las deformidades de tejido blando, en ambos planos y con magnitudes entre 11-30 mm. Las variables imagenológicas estudiadas mostraron un comportamiento interdependiente.

Expression of calpain system transcripts responds inversely to beef tenderization after vitamin D3 supplementation in Nellore cattle
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REZENDE, Lilian Ribeiro

; RAMOS, Patricia Maloso

; LOBO JUNIOR, Adalfredo Rocha

; GASPARIN, Gustavo

; JORGE, Erika Cristina

; MOURÃO, Gerson Barreto

; COUTINHO, Luiz Lehmann

; DELGADO, Eduardo Francisquine

Revista Brasileira de Saúde e Produção Animal

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2022, Volume 23 elocation e2122402021

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Uma vez que o sistema calpaína é central para o amaciamento da carne e dada a importância da atividade de calpastatina na determinação da maciez de bifes de bovinos Bos taurus indicus, o objetivo do presente estudo foi determinar se a expressão do gene de μ-calpaína (CAPN1), calpastatina total (CAST T), e suas variantes (CAST I e II) foi induzido pela inclusão de vitamina D3 na dieta. Os animais receberam nenhuma ou 2 × 106 UI dose de vitamina D3 por 2 ou 8 dias antes do abate, e foram submetidos a diferentes condições durante o confinamento: exposição solar ou sombreamento artificial. Bifes do Longissimus lumborum foram fabricados e submetidos a maturação por 1, 7, e 21 dias post-mortem e posteriormente usados para determinação da força de cisalhamento e do índice de fragmentação miofibrilar. Vitamina D3 não influenciou a abundância de RNAm, exceto para maior abundância de transcritos de CAST II em animais que foram suplementados por 8 dias antes do abate. Foi encontrada associação negativa entre a abundância de CAST II e a força de cisalhamento. Essa contradição revela uma importante modulação da expressão do sistema calpaína resultado da suplementação com vitamina D que pode ser importante na determinação de estratégias para melhorar a maciez da carne.

ABSTRACT The calpain system is the central player for meat tenderization and the calpastatin activity plays an important role in beef tenderness of Bos taurus indicus cattle. This study investigated whether dietary vitamin D3 induced gene expression of μ-calpain (CAPN1), total calpastatin (CAST T), and their variants (CAST I and II). Animals received none or 2 × 106 IU of vitamin D3 for either 2 or 8 days before slaughter and were submitted to different conditions during feedlot: sun exposure or artificial shade. Steaks from Longissimus lumborum were fabricated, aged for 1, 7, and 21 days post-mortem, and later used for the analyses of shear force and the myofibrillar fragmentation index. Vitamin D3 did not influence mRNA abundance; however, it induced a greater CAST II transcript in animals supplemented 8 days before slaughter. There was a negative association between CAST II abundance and the shear force, which revealed an important modulation of the calpain system expression due to vitamin D supplementation. This result is an important tool for strategies to improve beef tenderness.

https://doi.org/10.1590/s1519-99402122xx2021

Beef of Nellore cattle has limited tenderization despite pH decline in Longissimus lumborum
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Ramos, Patricia Maloso

; Santos-Donado, Priscila Robertina dos

; Oliveira, Giuliana Micai de

; Contreras–Castillo, Carmen Josefina

; Scheffler, Tracy L.

; Silva, Saulo da Luz e

; Martello, Luciane Silva

; Delgado, Eduardo Francisquine

Scientia Agricola

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2022, Volume 79 Nº 3 elocation e20200340

Resumo: En | Texto: En | PDF: En

ABSTRACT: Bos taurus indicus temperament is variable and affects beef tenderization. Our objective was to investigate temperament and performance of non–castrated Nellore and identify groups based on Longissimus lumborum (LL) pH decline as well as beef characteristics produced by those groups. We investigated 94 animals with a subset of carcasses (n = 24) selected based on LL pH at 24 h postmortem (pm) to represent two groups: resistant to pH decline (> 5.8 called pH–Res; n = 10) and normal (< 5.7 called pH–Nor; n = 14). Steaks were fabricated from the LL muscle and randomly assigned to aging (2, 7, 14, and 21 days). Sarcomere length, cooking loss, myofibrillar fragmentation index (MFI), and Warner–Bratzler shear force (WBSF) were determined. Data on temperament were investigated in a multivariate approach, while beef data were compared between groups using the analysis of variance. Rectal temperature at the beginning of the finishing phase and total weight gain were greater and related to animals in the pH–Res group (p < 0.01 and p = 0.03, respectively). Temperature and pH decline curves, sarcomere length, and cooking loss revealed that pH–Res produced beef with lower quality compared to the pH–Nor group. Results for MFI and WBSF did not show differences between groups within each time pm; however, overall steaks from pH–Res were tougher (p = 0.06). Incidence of LL pH between 5.8 and 5.9 at 24 h pm did not compromise the tenderization rate or extension; however, it affected the water holding capacity in this population of Nellore cattle.

https://doi.org/10.1590/1678-992x-2020-0340 176 downloads

Brazilian Nutritional Consensus in Hematopoietic Stem Cell Transplantation: Adults
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Barban, Juliana Bernardo

; Simões, Belinda Pinto

; Moraes, Bruna Del Guerra de Carvalho

; Anunciação, Cássia Rehem da

; Rocha, Cristiane Santos da

; Pintor, Daniela Cristina Querino

; Guerra, Daiane Cristina

; Silva, Deise Andrade

; Brandão, Edith de Castro Martins

; Kerbauy, Fábio

; Pires, Fernanda Ramos de Oliveira

; Morais, Graciele Lima

; Schmidt Filho, Jayr

; Sicchieri, Juliana Maria Faccioli

; Barroso, Karine Sampaio Nunes

; Viana, Luciana Verçoza

; Rocha, Mariana Hollanda Martins da

; Guimarães, Maysa Penteado

; Lazzari, Natalia Leonetti Couto

; Hamerschlak, Nelson

; Ramos, Patricia

; Gomes, Plinio Nascimento

; Mendonça, Priscila da Silva

; Oliveira, Raquel Candido de

; Scomparim, Renata Corrêa

; Chiattone, Ricardo

; Diez-Garcia, Rosa Wanda

; Cardenas, Thaís de Campos

; Miola, Thais Manfrinato

; Costa, Thalita Cristina de Mello

; Rocha, Vanderson

; Pereira, Andrea Z

einstein (São Paulo)

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2020, Volume 18 elocation AE4530

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O estado nutricional do paciente submetido ao transplante de células-tronco hematopoéticas é considerado fator de risco independente, podendo influenciar na qualidade de vida e na tolerância ao tratamento proposto. O comprometimento do estado nutricional durante o transplante de células-tronco hematopoéticas ocorre principalmente devido aos efeitos adversos decorrentes do condicionamento ao qual o paciente é submetido. Desta forma, a adequada avaliação nutricional e o acompanhamento durante o transplante de células-tronco hematopoéticas tornam-se imprescindíveis. Com o objetivo de salientar a importância do estado nutricional e da composição corporal durante o tratamento, bem como as principais características relacionadas à avaliação nutricional do paciente, o Consenso Brasileiro de Nutrição em Transplante de Células-Tronco Hematopoiéticas: Adulto foi elaborado visando uniformizar e atualizar a Terapia Nutricional nesta área. Com a participação de nutricionistas, nutrólogos e hematologistas de 15 centros brasileiros referências em transplante de células-tronco hematopoéticas

ABSTRACT The nutritional status of patients submitted to hematopoietic stem cell transplant is considered an independent risk factor, which may influence on quality of life and tolerance to the proposed treatment. The impairment of nutritional status during hematopoietic stem cell transplant occurs mainly due to the adverse effects resulting from conditioning to which the patient is subjected. Therefore, adequate nutritional evaluation and follow-up during hematopoietic stem cell transplant are essential. To emphasize the importance of nutritional status and body composition during treatment, as well as the main characteristics related to the nutritional assessment of the patient, the Brazilian Consensus on Nutrition in Hematopoietic Stem Cell Transplant: Adults was prepared, aiming to standardize and update Nutritional Therapy in this area. Dietitians, nutrition physicians and hematologists from 15 Brazilian centers thar are references in hematopoietic stem cell transplant took part.

https://doi.org/10.31744/einstein_journal/2020ae4530 3888 downloads

Cold shortening decreases the tenderization of Biceps femoris muscle from lambs
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FAUSTO, Daiane Aparecida

; LIMA, Marcella Arcaro de

; RAMOS, Patricia Maloso

; PERTILE, Simone Fernanda Nedel

; SUSIN, Ivanete

; DELGADO, Eduardo Francisquine

Revista Brasileira de Saúde e Produção Animal

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Mar 2017, Volume 18 Nº 1 Páginas 16 - 25

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Foram avaliados os efeitos do encurtamento normal (NS) e encurtamento pelo frio (CS) no comprimento do sarcômero, força de cisalhamento pelo Warner-Bratzler (WBS) e no processo de amaciamento analisado pelo índice de fragmentação miofibrilar (MFI). Os músculos Bicep femoris de dez ovinos oriundos do cruzamento Dorper x Santa Inês, foram aleatoriamente designados para: 1) NS: resfriamento do músculo na carcaça por 24 horas na câmara fria a 4°C (NS) 2) CS: resfriamento rápido, em banho de gelo, do músculo removido a partir da carcaça imediatamente após o abate (CS). Comprimento de sarcômero foi determinado nas amostras coletadas 24 horas após o abate e WBS e MFI nos períodos de maturação (1 ou 10 post mortem), em cada tratamento. Os valores de WBS da carne para 1 e 10 dias foram maiores (P <0,05) para CS (4,90 ± 0,42 e 3,73 ± 0,42 kgf, respectivamente) que para NS (4,07 ± 0,36 e 2,98 ± 0,38 kgf, respectivamente). Houve correlação negativa para WBS e comprimento do sarcômero (r = −0,59 e r = −0,69, P <0,05) nos dias 1 e 10 de post-mortem. Além disso, foram observadas correlações positivas entre MFI e comprimento do sarcômero (r = 0,46 e r = 0,48, P <0,05) nos dias 1 e 10, respectivamente. Esses resultados apontam para um impacto negativo do encurtamento do sarcômero no processo de amaciamento de carne em músculo do pernil de cordeiros.

SUMMARY This study evaluates the effects of Normal Shortening (NS) and Cold Shortening (CS) in sarcomere length, Warner-Bratzler shear force (WBS) and tenderization process examined by Myofibrillar Fragmentation Index (MFI). The Biceps femoris muscle from ten Dorper x Santa Inês lambs was randomly assigned to 1) NS = cooling of the muscle in the carcass for 24 hours in a cold room at 4°C; or 2) CS = rapid cooling, in ice bath, of the muscle removed from the carcass immediately after slaughter. Sarcomere Length (SL) was determined in the collected samples 24 hours after slaughter and WBS and MFI in the aging periods (1 or 10 days post-mortem). The WBS value of the meat for 1 and 10 days were greater (P<0.05) for CS (4.90 ± 0.42 and 3.73 ± 0.42 kgf, respectively) than NS (4.07 ± 0.36 and 2.98 ± 0.38 kgf, respectively). There were negative correlations between WBS and SL (r= −0.59 and r=−0.69, P< 0.05) at 1 and 10 postmortem, respectively. Furthermore, there were positive correlations for MFI and SL (r= 0.46 and r=0.48, P< 0.05) at days 1 and 10, respectively. These results point to a negative impact of sarcomere cold shortening in the sheep meat tenderization process in the hindlimb muscle.

https://doi.org/10.1590/s1519-99402017000100003 1771 downloads

Behavioral alterations induced by repeated saxitoxin exposure in drinking water
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Diehl, Felipe

; Ramos, Patricia Baptista

; Santos, Juliane Marques dos

; Barros, Daniela Martí

; Yunes, João Sarkis

Journal of Venomous Animals and Toxins including Tropical Diseases

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2016, Volume 22 elocation 18

Resumo: En | Texto: En | PDF: En

Abstract Background Blooms of the saxitoxin-producing cyanobacterium Cylindrospermopsis raciborskii have been contaminating drinking water reservoirs in Brazil for many years. Although acute effects of saxitoxin intoxication are well known, chronic deleterious outcomes caused by repeated saxitoxin exposure still require further investigation. The aim of the present work is to investigate the effects of consumption of drinking water contaminated with C. raciborskii for 30 days on learning and memory processes in rats. Methods The effects of saxitoxin (3 or 9 μg/L STX equivalents) or cyanobacteria on behavior was determined using the open field habituation task, elevated plus maze anxiety model task, inhibitory avoidance task, and referential Morris water maze task. Results No effects of saxitoxin consumption was observed on anxiety and motor exploratory parameters in the elevated plus maze and open field habituation tasks, respectively. However, groups treated with 9 μg/L STX equivalents displayed a decreased memory performance in the inhibitory avoidance and Morris water maze tasks. Conclusions These results suggest an amnesic effect of saxitoxin on aversive and spatial memories.

https://doi.org/10.1186/s40409-016-0072-9 1636 downloads

¿Nuevo rol para los territorios en Cuba? Avances y contradicciones en el contexto actual
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Ramos, Patricia

; Iñiguez, Luisa

GOT, Revista de Geografia e Ordenamento do Território

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Dez 2015, Nº 8 Páginas 191 - 215

Resumo: Es Pt En | Texto: Es Pt En | PDF: Es | PDF: Pt | PDF: En

El contexto vigente en Cuba, promueve una nueva comprensión del territorio no solo como soporte sino como agente de los cambios que se emprenden. El artículo recrea algunas de las transformaciones que han tenido lugar en la economía cubana desde una perspectiva territorial. Se muestra, de conjunto con los avances indiscutibles, la presencia de un entorno hasta cierto punto contradictorio para el desarrollo de las potencialidades de los territorios en Cuba. De un lado florece un discurso político favorable y del otro normas y regulaciones institucionales que como parte del proceso de cambios actuales apuntan a desaprovechar las oportunidades de los territorios en su ascenso económico y social.

A situação atual em Cuba promove uma nova compreensão do território, não só como um instrumento de apoio mas também como um agente das transformações que são realizadas. O artigo reproduz algumas das alterações ocorridas na economia cubana partir de uma perspetiva territorial, demonstrando, juntamente com o inegável progresso, a presença de um ambiente algo contraditório, relativamente ao desenvolvimento do potencial dos territórios em Cuba. Por um lado, desenvolve um discurso político favorável e por outro lado, algumas normas e regulamentos institucionais, como parte das mudanças atuais, levam a desperdiçar as oportunidades dos territórios no seu avanço económico e social.

The current context in Cuba, promotes a new understanding of the territory not only as a support but as an agent of transformations that are undertaken. The article recreates some of the changes that have taken place in the Cuban economy from a territorial perspective. It shows, in conjunction with the undeniable progress, the presence of an environment somewhat contradictory for the development of the potential of the territories in Cuba. On the one hand flourishes a favorable political speech and in the other hand, some institutional rules and regulations as part of the current changes aim to misspend the opportunities of territories in their economic and social advancement.

https://doi.org/10.17127/got/2015.8.010 1094 downloads

10.

Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts
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Oliveira, Lilian C.G.

; Ramos, Patricia Locosque

; Marem, Alyne

; Kondo, Marcia Y.

; Rocha, Rafael C.S.

; Bertolini, Thiago

; Silveira, Marghuel A.V.

; Cruz, João Batista da

; Vasconcellos, Suzan Pantaroto de

; Juliano, Luiz

; Okamoto, Debora N.

Brazilian Journal of Microbiology

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Jun 2015, Volume 46 Nº 2 Páginas 347 - 354

Resumo: En | Texto: En | PDF: En

Halophilic microorganisms are able to grow in the presence of salt and are also excellent source of enzymes and biotechnological products, such as exopolysaccharides (EPSs) and polyhydroxyalkanoates (PHAs). Salt-tolerant bacteria were screened in the Organic Composting Production Unit (OCPU) of São Paulo Zoological Park Foundation, which processes 4 ton/day of organic residues including plant matter from the Atlantic Rain Forest, animal manure and carcasses and mud from water treatment. Among the screened microorganisms, eight halotolerant bacteria grew at NaCl concentrations up to 4 M. These cultures were classified based on phylogenetic characteristics and comparative partial 16S rRNA gene sequence analysis as belonging to the genera Staphylococcus, Bacillus and Brevibacterium. The results of this study describe the ability of these halotolerant bacteria to produce some classes of hydrolases, namely, lipases, proteases, amylases and cellulases, and biopolymers. The strain characterized as of Brevibacterium avium presented cellulase and amylase activities up to 4 M NaCl and also produced EPSs and PHAs. These results indicate the biotechnological potential of certain microorganisms recovered from the composting process, including halotolerant species, which have the ability to produce enzymes and biopolymers, offering new perspectives for environmental and industrial applications.

https://doi.org/10.1590/S1517-838246220130316 2946 downloads

11.

Pork loin two-toning and drip loss in relation to steak cross-section anatomical position, plasma and exudate glucose
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Ramos, Patricia Maloso

; Delgado, Eduardo Francisquine

Scientia Agricola

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Ago 2014, Volume 71 Nº 4 Páginas 266 - 273

Resumo: En | Texto: En | PDF: En

Perception of color and its relationship to water holding capacity are important for defining the yield and quality of the pork production process. The aim of this study was to identify the relationship among color measurements taken at various anatomical positions in the cross-sectional surface of pork loin steak, and measurements of fluid exudation and its glucose concentration, as well as the impact on these attributes due to plasma glucose at slaughter. Two assays were conducted sequentially: i) investigation of the surface color parameters at different anatomical positions in the pork loin cross-section and their relationship to general exudation; and ii) the effect of plasma glucose levels on surface color variables, drip loss and glucose exudate concentration in three anatomical regions in the steak. The L* value of the ventro-lateral region, in the first assay, had the highest correlation with average steak drip loss at all anatomical points, exudation increasing proportionally between 48 and 72 h. The hue angle was also positively correlated with drip loss and lightness. The drip loss was greater when the animals had high plasma glucose, especially in the intermediate and lateral regions of the steak surface. The intermediate region presented greater lightness and lower redness. The plasma and glucose exudate concentrations, potential indicators of the muscle glycolytic metabolism, were related to color and drip loss. These variables can be influenced by the anatomical region inside the muscle, impacting the ability to retain water, two-toning occurrence and overall pork loin quality.

https://doi.org/10.1590/0103-9016-2013-0186 2220 downloads

12.

Diseño de una crema regeneradora con quitina para después del bronceado
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Pérez Ramos, Patricia

; Nieto Acosta, Olga María

; Bilbao Reboredo, Ofelia

; López Torres, Ariana

; González Cabrer, Lenia

Revista Cubana de Farmacia

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Jun 2013, Volume 47 Nº 2 Páginas 238 - 251

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Introduction: after taking the sun, it is indispensable to repair the skin affected by harmful rays. The cosmeceuticals for use after sunbath have antinflammatory, relaxing, hydrating, soothing and regenerative elements of the epithelium, which prevent or mitigate the feeling of pain, tautness, burning and rash. Objective: to develop a regenerative chitin cream to be used after sun tanning, with due quality to repair and hydrate the skin. Methods: two experimental designs 2² (F1 and F2) were created in which glycerin and propilenglycol were used as humectants respectively. Ph and extensibility were measured as variables in addition to analyzing the psychophysiological properties, the shearing tension and the centrifugation tests as well as the temperature impact during 60 days. Results: there were no significant differences between propilenglycol and glycerin whereas the incidence of cetyl alcohol in extensibility and pH values was noticeable. The best variant was the no. 1 in the F2 design in which the cetyl alcohol was found at 4 % and the propilenglycol at 3 %. The pH value was 7.05 ± 0.00; the extensibility was 68.15 ± 1.51 and the shear tension was 1.93 ± 0.01. The psychophysiological properties were satisfactory and there was no creaming or coalescence. Conclusions: it may be considered that, according to the measurements made in the evaluated period of time, the characteristics are satisfactory for the prepared creams; being variant I of the F2 design the least expensive. The physical stability of formulations undergoing tests of centrifugation and temperature impact indicates good compatibility between the studied polymer and the excipients.

Introducción: después de tomar el sol resulta indispensable reparar la piel agredida por los rayos nocivos. Los cosmecéuticos para después del sol contienen elementos antinflamatorios, calmantes, hidratantes, suavizantes y regeneradores del epitelio, los cuales evitan o mitigan la sensación de dolor, tirantez, ardor y prurito. Objetivo: desarrollar una crema regeneradora con quitina para después del bronceado con la calidad requerida para reparar e hidratar la piel. Métodos: se realizaron dos diseños experimentales 2² (F1 y F2), en los que se utilizaron humectantes diferentes, glicerina y propilenglicol respectivamente. Se midieron como variables respuestas: el pH y la extensibilidad; además se analizaron las propiedades psicofisiológicas, la tensión de cizalla y las pruebas de centrifugación e influencia de la temperatura durante 60 días. Resultados: no existieron diferencias significativas entre el uso del propilenglicol o la glicerina, mientras que si fue notable la incidencia del alcohol cetílico en los valores de extensibilidad y pH. Se obtuvo como la mejor variante la I del diseño F2, en la que el alcohol cetílico se encontraba al 4 % y el propilenglicol al 3 %; el pH resultó 7,05 ± 0,00; la extensibilidad igual a 68,15 ± 1,51 y la tensión de cizalla de 1,93 ± 0,01; las propiedades psicofisiológicas resultaron satisfactorias y no hubo cremado ni coalescencia. Conclusiones: se puede considerar, atendiendo a las mediciones realizadas en el tiempo evaluado, características satisfactorias para las cremas elaboradas siendo la de menor costo, la variante I del diseño F2. La estabilidad física de las formulaciones, sometidas incluso las bases a pruebas de centrifugación e influencia de la temperatura, es indicativo de una buena compatibilidad entre el biopolímero estudiado y los excipientes de la formulación.

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13.

A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia
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Gallo, Stephanie Wagner

; Ramos, Patricia Locosque

; Ferreira, Carlos Alexandre Sanchez

; Oliveira, Silvia Dias de

Memórias do Instituto Oswaldo Cruz

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Mai 2013, Volume 108 Nº 3 Páginas 390 - 391

Resumo: En | Texto: En | PDF: En

Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.

https://doi.org/10.1590/S0074-02762013000300020 3431 downloads

14.

Diseño y desarrollo de dos mascarillas faciales para el acné con quitina como sustancia bioactiva
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Pavón Pérez, Jessy

; Valdés Comas, Leslie

; Pérez Ramos, Patricia

Revista Cubana de Farmacia

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Jun 2011, Volume 45 Nº 2 Páginas 251 - 263

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

The cosmetics industry attaches importance to the achievement of a more complete cosmetic, a cosmoceutic representing safety in health and beauty maintenance. In present paper were designed and developed two face packs for acne using 2 % chitin as active substance. This polymer of marine origin has phylmogen and hydration properties and a demonstrated healing and antimicrobial action. Two experimental designs were carried out with a D-optimal mixture and also the study of incidence of three components of both face packs, polyquaaterium-7 (salcare SC91®) in one of the variants be assayed and sodium cellulose carboxymethyl (SCC) in the other one, 2 % propylene glycol and 91.6 % water) in the case of salcare formula and the 5 variant (2 % SCC, 4 % propylene glycol and 89.6 % water) in SCC formula.

La industria cosmética brinda importancia al logro de un cosmético más completo, un cosmecéutico, que represente seguridad en el mantenimiento de la salud y la belleza. En este trabajo se diseñaron y desarrollaron 2 mascarillas faciales para el acné con quitina al 2 % como sustancia activa. Este polímero de origen marino posee propiedades hidratantes, filmógenas, ha sido demostrada su acción cicatrizante y antimicrobiana. Se realizaron 2 diseños de experimentos con mezcla D-optimal, y se estudió la incidencia de 3 componentes en ambas mascarillas: polyquaternium-7 (salcare SC91®) en una de las variantes a ensayar y carboximetil celulosa sódica (CMC) en la otra, propilenglicol y agua en ambos casos. Se analizaron en el tiempo el pH y la extensibilidad; resultó óptima la variante 4 del diseño (salcare SC91 2 %, propilenglicol 2 % y agua al 91,6 %) en el caso de la formulación con salcare y la variante 5 (CMC 2 %, propilenglicol 4 % y agua 89,6 %) en la formulación con CMC.

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15.

LA PARÁLISIS CEREBRAL INFANTIL: SU IMPACTO SOBRE EL AJUSTE EMOCIONAL Y DESEMPEÑO SOCIAL DE LA MADRE
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Pérez Álvarez, Luis

; Mendiondo Ramos, Patricia

Revista Archivo Médico de Camagüey

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Out 2007, Volume 11 Nº 5

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

A cross-sectional descriptive study with the objective to assess the behavior of emotional and social factors in 30 mothers of children carriers of infintile paralysis was carried out. The 100% of the interviewees referred that child´s disease constituted a serious problem, in a 73.33% affected the emotional adjustment, the 90.47% of the ones who worked stop doing it. By applying Dembo Rubinstein autovaluation scale, the 100% was below the midpoint of the scale of happiness and referred character changes. In the psychometric test of ten desires the 100% considered as their most important desire was to see their son improve. In applying the R B Catell Autoanlysis Model the 100% a high level of anxiety was appreciated. It is important to investigate on how the disability in offspring affects parents to implement measures that would help them to face this situation.

Se realizó un estudio descriptivo transversal con el objetivo de evaluar el comportamiento de factores emocionales y sociales en 30 madres de niños portadores de parálisis cerebral infantil. El 100 % de las entrevistadas refirió que la enfermedad del hijo constituyó un serio problema, en un 73.33 % afectó el ajuste emocional, el 90.47 % de las que trabajaban depararon de hacerlo. Al aplicarles la escala de autovaloración de Dembo Rubinstein el 100 %, se situó por debajo del punto medio de la escala de felicidad y refirieron cambios de carácter. En la prueba psicométrica de los diez deseos el 100 % consideró como su deseo más importante era ver a su hijo mejorar. Al aplicar el Modelo de Autoanálisis de R.B Catell en el 100 % se apreció un elevado nivel de ansiedad. Es importante investigar sobre cómo la discapacidad en la descendencia afecta a los padres para implementar medidas que permitan ayudarlos a enfrentar esa situación.

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